There are people who cultivate psilocybin spores and depend on the surrounding environment which consists of: soil conditions, temperature, light, competition for resources, diseases, the existence of pests and the level of humidity. Variables can be easily controlled in a laboratory. Making beneficial changes to the environment is manageable as it has been shown in many cultivation studies made to guide mushroom growers. The best place to buy mushroom spores from industry leaders for research is Shaman Mushroom Spores.

The compost can be kept in a sealed bag at room temperature for up to three years before beginning cultivation process. However, contamination of the substrate can occur overtime and the viability of the spores can be severely impacted.

There is a lot of research stating that adding tryptamine to the environment can prove beneficial to the cultivation process. Moreover, mushrooms do better in the shade. If they are subjected to bright, dim or indirect light, they become less potent. The ones that live in the dark have the highest potency, whereas the minimum levels of substance are, usually, found in those exposed to the light. Exposure to the sun (or to light in general) reduces the amount of active substance in the mushrooms.

Finally, the harvesting process is to be handled carefully so as not to jeopardize the entire work and result in failure or waste.